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imaris isosurface function  (Oxford Instruments)
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Oxford Instruments imaris isosurface function
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Imaris Isosurface Function, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isosurface reconstructions  (Oxford Instruments)
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Oxford Instruments isosurface reconstructions
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Isosurface Reconstructions, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isosurface analysis features  (Oxford Instruments)
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Oxford Instruments isosurface analysis features
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Isosurface Analysis Features, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d isosurfaces detection  (Oxford Instruments)
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Oxford Instruments 3d isosurfaces detection
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
3d Isosurfaces Detection, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isosurface rendering tool  (Oxford Instruments)
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Oxford Instruments isosurface rendering tool
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Isosurface Rendering Tool, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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isosurfaces  (Oxford Instruments)
99
Oxford Instruments isosurfaces
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Isosurfaces, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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imaris isosurface  (Oxford Instruments)
99
Oxford Instruments imaris isosurface
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Imaris Isosurface, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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analysis isosurface  (Oxford Instruments)
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Oxford Instruments analysis isosurface
Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies <t>isosurface</t> after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).
Analysis Isosurface, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies isosurface after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).

Journal: bioRxiv

Article Title: Spatial growth in food-like matrices differentially modulates food-related stress responses but enhances digestive tolerance in major foodborne pathogens

doi: 10.64898/2026.03.05.710002

Figure Lengend Snippet: Microcolonies architecture in GSFM (▴) or S-GSFM (●), after 96h at 25°C. A : Macroscopic images are obtained using an automated robot that integrates incubation and high-resolution imaging. Microscopic observations of microcolonies, endpoint stained with the cell-permeant nucleic acid dye Syto 61, are obtained with confocal laser scanning microscopy. Representative pictures correspond to the microcolonies isosurface after fluorescence segmentation using the Imaris isosurface functions. B : Quantitative parameters extracted from the isosurface of microcolonies in Imaris software. Each point represents an independent replicate (n=9), bars correspond to the mean of volume of microcolonies log 10 (µm 3 ) and sphericity (A.U.). A two-way ANOVA was performed to assess the effect of food stress. When a significant effect of the environment was detected, the Bonferroni multiple comparisons test was applied to evaluate the level of significance. Asterisks indicate significant differences between environments (p<0.05).

Article Snippet: Raw fluorescence of each image was then binarised using the automated Imaris isosurface function.

Techniques: Incubation, Imaging, Staining, Confocal Laser Scanning Microscopy, Fluorescence, Software